BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Talks.cam//talks.cam.ac.uk// X-WR-CALNAME:Talks.cam BEGIN:VEVENT SUMMARY:Coordinated alternative splicing in development\, featuring Transf ormer2b and ciliary tissues.-Dr. Charlotte Softley. - Dr. Holliday Lovegro ve\, Univ of Manchester &\; Dr. Charlotte Softley\, University Clinic F reiburg DTSTART:20231030T143000Z DTEND:20231030T153000Z UID:TALK206218@talks.cam.ac.uk CONTACT:Jia CHEN DESCRIPTION:Charlotte Softley: Coordinated alternative splicing in develop ment\, featuring Transformer2b and ciliary tissues\n\nCiliopathies are con ditions\, often systemic\, caused by disruptions in cilia formation or fun ction. They can affect any number of the ciliated cell types and tissues\, including multiciliated cells (eg lungs or fallopian tubes)\, motile mono -ciliated cells in the left-right organiser and primary cilia\, and signal ling. These conditions cause suffering in patients around the globe\; a be tter understanding of their causes will give us insight into potential tre atments.\n\nUnsurprisingly\, many components of the cilia and centrosome a re implicated in ciliopathies. However\, many mutations in spliceosome com ponents are also found to cause ciliopathies\, indicating that splicing al so plays an important role in ciliogenesis.\n\nInvestigating the alternati ve splicing factor Transformer2b (Tra2b) and its targets via RNAseq in the Xenopus laevis frog embryo\, we found that knockdown of Tra2b leads to di fferential splicing in more than a hundred proteins\, of which more than 3 0% are cilia-related. Dissecting these results and further analysis of flu id flow\, left-right patterning and signalling\, we found a seemingly conc erted action of Tra2b targets in ciliogenesis and cilia function. I will p resent our findings and draw links with the alternative splicing factor NO VA to question whether coordinated alternative splicing could play a role in tissue- and stage-specific development across organisms. LOCATION:Online END:VEVENT END:VCALENDAR