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SUMMARY:Deep screening of RNA\, XNA and protein interactions - Philipp Hol
 liger (MRC Laboratory of Molecular Biology)
DTSTART:20231121T170000Z
DTEND:20231121T180000Z
UID:TALK208588@talks.cam.ac.uk
CONTACT:Pietro Lio
DESCRIPTION:I will present deep screening\, an ultra-high-throughput appro
 ach leveraging the Illumina HiSeq platform for massively parallel sequenci
 ng\, display\, and affinity and kinetics screening at the level up to 109 
 individual RNA\, XNA and protein interactions. Deep screening enabled the 
 rapid discovery of 2-O-methyl-RNA aptamers as well as high-affinity nanobo
 dies (VHH) from immunized-alpaca or yeast display-enriched VHH libraries a
 nd single-chain Fv (scFv) antibody leads directly from unselected\, synthe
 tic scFv repertoires in a three-day experiment. Deep screening generates l
 arge\, internally-consistent genotype x phenotype (sequence x binding affi
 nity / kinetics etc.) correlation datasets that can serve as training data
  for a language-model-enabled machine learning approaches for the rapid in
  silico generation of novel sequences with even higher affinities. Deep sc
 reening promises to accelerate biomolecular discovery for a wide range of 
 modalities and targets.\n\nHYBRID TALK\nZOOM LINK:\nhttps://cl-cam-ac-uk.z
 oom.us/j/83721191406?pwd=V0xkY0Vvb3czWUJkR0xIZC9qMnM0QT09\n
LOCATION:Lecture Theatre 2\, Computer Laboratory\, William Gates Building
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