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SUMMARY:Insights into the mechanism of hind limb initiation in Xenopus lae
 vis  - John Young\, Department of Biology\, Simmons University
DTSTART:20240219T143000Z
DTEND:20240219T153000Z
UID:TALK210907@talks.cam.ac.uk
CONTACT:Jia CHEN
DESCRIPTION:*Abstract*: The vertebrate limb has provided a deep understand
 ing of the cellular\, genetic\, and molecular mechanisms that generate an 
 appendage. The majority of limb experimentation has been in animals where 
 the limb forms early in embryogenesis. Yet\, several tetrapods\, most nota
 bly frogs\, form their limbs well after embryonic patterning and different
 iation have occurred. Surprisingly\, we know very little about the process
 es that direct limb formation in amphibian tadpoles since most limb resear
 ch in these animals has focused on regeneration. Here we use both molecula
 r methods and classical transplant experiments to investigate the earliest
  steps in Xenopus hindlimb initiation. We found that posterior lateral pla
 te mesoderm from the late neurula contributes to the hind limb and that th
 is tissue expresses Pitx1 and Tbx4 in the early tadpole\, both key genes i
 nvolved in limb initiation. Surprisingly\, Fgf10 \, a major contributor li
 mb formation in amniotes\, is not expressed until well after bud formation
 . These results suggest that\, unlike amniotes\, Fgf10 is dispensable for 
 bud formation. Single-cell sequencing confirmed these observations and rev
 ealed several factors consistent with cell motility and migration in the l
 imb. Histological analyses revealed the limb generating cells are mesenchy
 mal at stage 40 and appear to condense into a bud by stage 46. Together\, 
 these data suggest a model that resembles zebrafish fin formation whereby 
 the limb-forming mesenchyme is specified early. However\, bud formation oc
 curs several days later via cell migration and condensation. This work pre
 sents new insights into how limb development varies across tetrapods. \n\n
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LOCATION:Online
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