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SUMMARY:CRISPy Chickens-screening fate transitions from caudal epiblast to
  neural tube-Ashley Libby\; Coupling mitochondrial energy metabolism to br
 anching morphogenesis in the developing avian lung-Bezia Lemma. - Ashley L
 ibby\, Briscoe lab\,The Francis Crick Institute\; and Bezia Lemma\, Nelson
  lab\, Princeton University
DTSTART:20240226T143000Z
DTEND:20240226T153000Z
UID:TALK210913@talks.cam.ac.uk
CONTACT:Jia CHEN
DESCRIPTION:*Title: CRISPy Chickens-screening fate transitions from caudal
  epiblast to neural tube-Ashley Libby*\n\nAbstract: \n\nA remarkable aspec
 t of embryonic development is the coordinated emergence of multiple cell p
 opulations in dynamically changing tissues. For example\, neural tube deve
 lopment requires a flat structure of epiblast cells to generate a final cl
 osed neural tube structure\, that has genetically stratified progenitor do
 mains. This involves the physical folding and elongation of the tissue\, w
 hich positions cells within varying gradients of high Wnt/FGF in epiblast 
 to high retinoic acid in the closed neural tube.  At the same time\, withi
 n this rapidly changing environment\, transcription factor regulation defi
 nes end populations in a robustly programmed pattern. However\, despite an
  overall morphogenic understanding of this process\, the molecular mechani
 sms that specify and coordinate progenitor transitions between the epiblas
 t and neural tube remain ill-defined. \n\nI will talk about how my researc
 h investigates both changes in tissue architecture and cell fate decisions
  by pairing single-cell imaging and genetic screen techniques. I will show
  multi-photon images and videos of chick embryos to highlight how cells mo
 ve as the neural tube is closing\, focusing on a gradient of decreasing la
 teral cell movement as cells progress from the epiblast to neural tube. In
  complement\, I will also talk about the design of a small in vivo pooled 
 CRISPR screen in chick embryos\, to examine how intrinsically changing a c
 ell’s receptiveness to morphogenic signalling affects later acquired fat
 es. Here\, I will talk about how we found epigenetic/transcriptional regul
 ators that facilitate the rapid interpretation/response to the changes in 
 FGF and retinoic acid to genetically guide rapid fate specification. \n\nO
 verall\, this work highlights several strategies\, physical and epigenetic
 \, by which the embryo coordinates cell fate in a dynamic signalling envir
 onment to form robust patterning and thus functional tissues. \n\n*Title: 
 Coupling mitochondrial energy metabolism to branching morphogenesis in the
  developing avian lung-Bezia Lemma*\n\nAbstract: Energy metabolism at a mo
 lecular scale is required to fuel cellular activity that gives rise to tis
 sue morphogenesis. However it is unclear whether energy metabolism is patt
 erned during organogenesis in a way that influences or aligns with gene ex
 pression and mechanical forces. We have measured the spatial patterns of m
 itochondrial membrane density\, membrane potential\, and ATP concentration
  in the embryonic chicken lung. At this stage of development\, the chicken
  lung is comprised of tubes of simple columnar epithelium surrounded by a 
 loose mesenchyme. To generate the first several branches off the dorsal su
 rface of the primary bronchus\, subsets of epithelial cells undergo actomy
 osin-driven apical constriction. The first three of these branching events
  are highly stereotyped in time and location\; we therefore focused on thi
 s geometrically ideal system to study how energy metabolism relates to cha
 nges in tissue morphology. Our results reveal heightened mitochondrial ene
 rgy metabolism specifically in epithelial areas of initiating branches. Ad
 ditionally\, we measured oxygen consumption rates to set physical bounds o
 n the levels of total mitochondrial respiration. These findings establish 
 a connection between the patterning of energy metabolism and the morphogen
 esis of multicellular tissues. This work is funded in part by an NIH Direc
 tor’s Pioneer Award (HD111539) and the NSF PRFB Grant No. (2305831) \n\n
 Join Zoom Meeting \n\n\n"https://us06web.zoom.us/j/82089026611?pwd=L2FyclJ
 FL2lYR0J3SFBDbHQyUFp6UT09":URL\n\n  \n\nMeeting ID: 820 8902 6611 \n\nPass
 code: 038347 \n\n\n 
LOCATION:Online
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