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SUMMARY:Deep sequencing reveals differential expression of microRNAs in fa
 vorable versus unfavorable neuroblastoma - Sven Rahmann (TU Dortmund)
DTSTART:20100504T150000Z
DTEND:20100504T160000Z
UID:TALK21438@talks.cam.ac.uk
CONTACT:Florian Markowetz
DESCRIPTION:Small non-coding RNAs\, in particular microRNAs\, regulate fin
 e-tuning of\ngene expression and can act as oncogenes or tumor suppressor 
 genes.\nDifferential microRNA expression has been reported to be of functi
 onal\nrelevance for tumor biology. Using next-generation sequencing\, the\
 nunbiased and absolute quantification of the small RNA transcriptome is\nn
 ow feasible. Neuroblastoma is an embryonal tumor with highly variable\ncli
 nical course. We analyzed the small RNA transcriptomes of five\nfavorable 
 and five unfavorable neuroblastomas using SOLiD\nnext-generation sequencin
 g\, generating a total of 188\,000\,000 reads.\nMicroRNA expression profil
 es obtained by deep sequencing correlated well\nwith real-time PCR data. C
 luster analysis differentiated between\nfavorable and unfavorable neurobla
 stomas\, and the microRNA\ntranscriptomes of these two groups were signifi
 cantly different.\nOncogenic microRNAs of the miR17-92 cluster and the miR
 -181 family were\noverexpressed in unfavorable neuroblastomas. In contrast
 \, the putative\ntumor suppressive microRNAs\, miR-542-5p and miR-628\, we
 re expressed in\nfavorable neuroblastomas and virtually absent in unfavora
 ble\nneuroblastomas.  In-depth sequence analysis revealed extensive\npost-
 transcriptional miRNA editing. Of 13 identified novel miRNAs\, three\nwere
  further analyzed\, and expression could be confirmed in a cohort of 70 ne
 uroblastomas.\n\nHosted by Florian Markowetz.
LOCATION:Cancer Research UK Cambridge Research Institute\, Lecture Theatre
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