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SUMMARY:Combining Functional and Network Analyses to Dissect RAS1/MAPK Sig
 nalling - Dariel Ashton-Beaucage\, Institute for Research in Immunology an
 d Cancer\, University of Montreal\, Canada
DTSTART:20091210T110000Z
DTEND:20091210T120000Z
UID:TALK21622@talks.cam.ac.uk
CONTACT:M. Madan Babu
DESCRIPTION:The RAS1/MAPK pathway participates in a wide array of cellular
  processes including proliferation\, differentiation and survival. Also\, 
 oncogene driven activation of MAPK signalling is frequently associated to 
 the development and progression of cancer. The RAS1-triggered RAF-MEK-MAPK
  cascade that forms the backbone of this pathway is supported by a series 
 of scaffolding and regulatory molecules that help in modulating signal str
 ength and duration. Much of our present knowledge on the makeup of this pa
 thway has been assembled from successive genetic screening experiments con
 ducted in the fly and worm.\n\nIn order to circumvent the limitations asso
 ciated with traditional genetic screening techniques\, we conducted a RNAi
  based functional screening analysis to get a global picture of the RAS1/M
 APK regulatory network. We then used a set of secondary epistasis assays t
 o position these genes in relation to the core pathway components. Integra
 tion of these results with a protein interaction network analysis revealed
  distinct complexes acting at different steps in pathway function. We focu
 s on a potential RAF regulatory complex and on a large set of mRNA process
 ing components acting downstream of MEK. These findings add depth to the g
 rowing network of factors that feed into this pathway at different levels 
 and\, in particular\, identify  mRNA processing as an important factor in 
 RAS1/MAPK signal control.
LOCATION:Structural Studies Seminar Room\, MRC Laboratory of Molecular Bio
 logy\, Cambridge\, UK
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