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SUMMARY:In Vivo studies with Optical Tweezers - Prof. Lene B. Oddershede\,
  Niels Bohr Institute\, Univ. Copenhagen\, Blegdamsvej 17\, 2100 Copenhage
 n\, Denmark
DTSTART:20100115T140000Z
DTEND:20100115T150000Z
UID:TALK22249@talks.cam.ac.uk
CONTACT:Erika Eiser
DESCRIPTION:Optical tweezers are a very versatile nano-tool capable of mea
 suring forces and distances inside\nliving cells in a nearly non-invasive 
 manner. In order to use the technique to probe fundamental\nphysical chara
 cteristics of living cells it is crucial that the tweezers do not influenc
 e the physiological state of the investigated organism[1]\, and that the p
 otential temperature increase associated with optical trapping is not sign
 ificant. Both issues will be addressed in the talk\, the latter using a no
 vel lipid bilayer molecular sorting assay.\nOptical investigations of two 
 different biological systems will be presented: The motility of an\nindivi
 dual protein in the outer membrane of E. coli bacteria will be unraveled\,
  the observed mobility\nbeing caused not only by thermal motion both in ad
 dition by an active motion associated with the\nmetabolism of the organism
  [2\,3]. Connected to this\, we show that antibiotics and antimicrobial pe
 p-\ntides have a pronounced effect on single protein motility [4]. The sec
 ond biological system presented\nwill be an S. pombe yeast cell\, where th
 e diffusion patterns of naturally occurring lipid granules have\nbeen unco
 vered using optical trapping and single particle tracking\; the granules p
 erform anomalous\ndiffusion\, with subdiffusion being most predominant at 
 short time-lags\, and the biological functions\ngiving motility footprints
  at longer time-lags [5\,6].\n\n1. M.B. Rasmussen\, L.B. Oddershede\, H. S
 iegumfeldt\, Applied and Environmental Microbiology\, 74 (2008).\n2. L. Od
 dershede\, J.K. Dreyer\, S. Grego\, S. Brown\, K. Berg-Sørensen\, Biophys
 ical Journal\, 83\, 3152 (2002).\n3. T. Winther\, L. Xu\, K. Berg-Srensen\
 , S. Brown\, and L.B. Oddershede. Biophysical Journal\, Bio-physical Journ
 al\, 97\, 1 (2009).\n4. T. Winther and L.B. Oddershede\, Current Pharmaceu
 tical Biotechnology\,10\, 486-493 (2009).\n5. I.M. Tolic-Nørrelykke\, E.-
 L. Munteanu\, G. Thon\, L. Oddershede\, and K. Berg-Sørensen\, PRL\, 93\,
  078102 (2004).\n6. V. Tejedor\, O. Benichou\, R. Voituriez\, R. Jungmann\
 , F. Simmel\, C. Selhuber-Unkel\, L.B. Odder-\nshede\, and R. Metzler\, Bi
 ophysical Journal (2010).
LOCATION:Pippard Lecture Theatre\, Cavendish Laboratory
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