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SUMMARY:ChIP-Seq in six Drosophila species reveals a highly similar bindin
 g landscape for the developmental transcription factor Twist. - Alexander 
 Stark (IMP Vienna)
DTSTART:20100607T150000Z
DTEND:20100607T160000Z
UID:TALK22250@talks.cam.ac.uk
CONTACT:Florian Markowetz
DESCRIPTION:Gene expression during development is controlled by transcript
 ion factors that bind to cis-regulatory sequences. Transcription factors a
 nd their target genes are often highly conserved\, yet the evolutionary dy
 namics of their binding sites has remained unclear. Here\, we report the f
 irst systematic ChIP-seq analysis of a key developmental transcription fac
 tor across six Drosophila species at a wide range of evolutionary distance
 s. We find that the entire binding landscape for the mesodermal transcript
 ion factor Twist is highly similar across species. This includes high-occu
 pancy sites\, as well as over 10\,000 low-occupancy sites near the detecti
 on limit.\n\nWe find that conserved binding sites are clustered and strong
 ly correlate with sequence motifs for Twist and its partners\, permitting 
 the de novo discovery of these regulators. Conserved low-occupancy sites t
 end to be occupied at different developmental stages\, providing an explan
 ation for the high abundance of binding sites for many transcription facto
 rs and suggesting that transcription factors can bind to inactive enhancer
 s\, potentially marking or preparing them for future activation.\n\nHosted
  by Duncan Odom.
LOCATION:Cancer Research UK Cambridge Research Institute\, Lecture Theatre
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