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SUMMARY:Self-Assembling Enzyme Materials and Enzyme Engineering for Flow B
 iocatalysis - Kersten Rabe\, Karlsruhe Institute of Technology
DTSTART:20241205T120000Z
DTEND:20241205T010000Z
UID:TALK225265@talks.cam.ac.uk
CONTACT:Prof Ljiljana Fruk
DESCRIPTION:Applications employing catalysts in fluidic\, cascaded setup a
 re becoming industrially increasingly relevant. In order to also incorpora
 te biocatalysts in such setups\, efficient immobilization strategies are i
 n demand. Employing the genetically encoded\, covalent SpyTag/SpyCatcher (
 ST/SC) system\, we have recently developed all-enzyme hydrogels\, which ca
 n also be formulated into biocatalytic foams and be prepared from many enz
 ymes\, enabling the continuous conversion of a wide variety of substrates.
  In such carrier-free formulations the enzymes themselves form a material\
 , which for example stabilizes the enzymes. These and other similar immobi
 lization strategies result in biocatalytic flow reactors with continuous c
 onversions for several days with high efficiency.\nAs an alternative appro
 ach\, biocatalysts can also be implemented in flow biocatalysis employing 
 additive manufacturing. Such bioprinting applications call for thermotoler
 ant organisms and proteins. We have recently showed reported how to employ
  guided protein evolution for engineering biocatalysts for such applicatio
 ns. In this context new methods using correlation analysis and machine lea
 rning have been developed which greatly aid the identification of thermost
 able enzymes. Using these approaches\, we have successfully evolved enzyme
  variants that reveal significantly increased stability and activity at el
 evated temperatures\, which can be utilizes for direct 3D printing for on 
 demand production of reactor modules. Different enzyme classes have been s
 uccessfully utilized including alcohol dehydrogenases\, esterases\, ketois
 ovalerate decarboxylases\, phenolic acid decarboxylases and benzaldehyde l
 yases.\n
LOCATION:Chemical Engineering and Biotechnology\, LT 1
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