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SUMMARY:Dynamics of molecular clocks expose the lineage relations of cells
  - Shalev Itzkovitz (MIT)
DTSTART:20100517T150000Z
DTEND:20100517T160000Z
UID:TALK22551@talks.cam.ac.uk
CONTACT:Florian Markowetz
DESCRIPTION:Understanding the embryonic development and post-natal mainten
 ance in multi-cellular organisms requires advanced methods for cell lineag
 e reconstruction of individual cells. The mammalian female germline is a c
 lassic example in which the fundamental aspects of these processes remain 
 highly debatable\, with doubts as to the clonal relation between adult ste
 m cells and primordial germ cells\, as well as conflicting evidence of pos
 t-natal oocyte renewal. We developed a high-throughput method for reconstr
 ucting cell lineage trees from genomic variability caused by somatic mutat
 ions in microsatellite molecular clocks. We have reconstructed lineage tre
 es of hundreds of oocytes and other cell types\, sampled from mismatch-rep
 air deficient mice at various ages. Analysis of these trees sheds light on
  the lineage relations between oocytes and bone-marrow derived cells\, on 
 the nature of the migration-expansion process of primordial germ cells and
  on the dynamics of oocyte aging.\n\nHosted by Nitzan Rosenfeld.
LOCATION:Cancer Research UK Cambridge Research Institute\, Lecture Theatre
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