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SUMMARY:A quantitative view of gene expression levels and epigenetic modif
 ications - Sarah Teichmann (University of Cambridge)
DTSTART:20100621T150000Z
DTEND:20100621T160000Z
UID:TALK22552@talks.cam.ac.uk
CONTACT:Florian Markowetz
DESCRIPTION:Gene expression levels are believed to be continuously distrib
 uted from very low to very high levels\, with most genes at an intermediat
 e level. We have studied the transcriptome-wide distribution of expression
  levels in T helper cells based on microarray and RNA-seq technology. We i
 ntegrated this data with single molecule mRNA-fish experiments for well-ch
 aracterized genes\, allowing the quantification of mRNA transcripts at sin
 gle-molecule resolution. The results of these analyses and experiments sho
 w that virtually all genes are expressed at > ~ 1 molecule per cell and th
 at two major expression levels can be identified which vary by roughly one
  to two orders of magnitude\, giving rise to strongly bimodal expression d
 istributions. Analysis of histone modifications by ChIP-seq indicates that
  activating modifications such as H3K9/14ac and H3K4me3 are involved in th
 is ‘digital’ expression switch. Our findings have broad implications f
 or the analysis of microarray\, RNA-seq and ChIP-seq data and for the unde
 rstanding of the regulation of gene expression.\n\nHosted by Florian Marko
 wetz.
LOCATION:Cancer Research UK Cambridge Research Institute\, Lecture Theatre
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