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SUMMARY:Joint ChemBio and Synthesis RIG Seminar - Chemical Biology Tools f
 or Measuring Drug Delivery - Joshua Kritzer (Tufts University)
DTSTART:20250523T130000Z
DTEND:20250523T140000Z
UID:TALK229642@talks.cam.ac.uk
CONTACT:Jasmine Mitchell
DESCRIPTION:Large-molecule therapeutics including peptides\, oligonucleoti
 des\, and proteins make up a large and growing portion of the drug develop
 ment pipeline.  One of the greatest barriers to developing these drugs is 
 cell penetration. Most enter the cell through a complex pathway involving 
 endocytosis followed by endosomal escape. This process is so poorly unders
 tood and difficult to study that it is challenging simply to measure how m
 uch compound has actually accessed the cytosol at any given point.  The Kr
 itzer Lab has developed new tools for making these and related measurement
 s.  The Chloroalkane Penetration Assay (CAPA) is a versatile assay that me
 asures cell penetration using cellularly expressed HaloTag protein and a s
 mall chloroalkane tag on the molecule-of-interest.  CAPA has been used by 
 the Kritzer group to measure cell penetration for many classes of peptide 
 and oligonucleotide therapeutics\, to measure penetration to different sub
 cellular compartments\, and to measure relative penetration in different c
 ell types.  CAPA has also been adopted by academic and industrial groups a
 ll over the world to investigate cell penetration.  The Kritzer group has 
 also used molecular evolution to produce new HaloTag variants which work o
 ptimally with a fluorogenic benzothiadiazole dye.  The resulting “BenzoT
 ag” system allows for turn-on\, no-wash cell labeling in seconds. BenzoT
 ag is currently being applied to produce a “turn-on” version of CAPA f
 or continued investigation of drug delivery and mechanisms of endosomal es
 cape
LOCATION:Dept. of Chemistry\, Wolfson Lecture Theatre
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