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SUMMARY:Condensing the Message: How Notch Signaling Forms Transcriptional 
 Hubs to Control Gene Activation - Carmen Santa Cruz Mateos. Department of 
 Physiology Development and Neuroscience\, University of Cambridge.
DTSTART:20250716T150000Z
DTEND:20250716T163000Z
UID:TALK233431@talks.cam.ac.uk
CONTACT:Daniel Sobrido-Cameán
DESCRIPTION:Developmental decisions rely on cells making accurate transcri
 ptional responses to signals they receive. For example\, Notch pathway act
 ivity results in rapid transcriptional outputs in the absence of any ampli
 fication steps. Local condensates or transcription factor hubs are propose
 d to facilitate recruitment of key nuclear complexes and their co-factors 
 to promote gene activation. To investigate whether transcription hubs are 
 formed under conditions of endogenous Notch signalling\, we combined real-
 time measurements of Notch transcription-complex enrichments relative to a
  fluorescently tagged gene locus with quantitative live imaging of gene tr
 anscription from two linked loci. An enriched hub containing the co-activa
 tor Mastermind (Mam) was formed in a signalling-dependent manner during de
 velopmental stages when transcription occurs. Tracking hubs in real time r
 evealed that they are highly dynamic and\, when imaged together with trans
 cription in the same nucleus\, Mam condensation consistently correlates wi
 th the onset and profile of transcription. Manipulations affecting signall
 ing levels had concordant effects on hub intensities and transcriptional p
 rofiles\, altering the probability and amplitude of transcription. Togethe
 r the results support a model in which signalling induces the formation of
  transcription hubs whose properties are instrumental in the quantitative 
 gene expression response to Notch activation.
LOCATION:in person at Gurdon Institute
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