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SUMMARY:Synthesis and Sequencing of Sequenced-Defined Biotic and Abiotic P
 olymers - Professor Eric Anslyn - The University of Texas at Austin
DTSTART:20251023T130000Z
DTEND:20251023T140000Z
UID:TALK238885@talks.cam.ac.uk
CONTACT:Sharon Connor
DESCRIPTION:There is little argument that many of the grand achievements o
 f biotechnology\, biochemistry\, and chemical biology stem from advances i
 n synthetic organic chemistry embodied in the development of solid-phase s
 ynthetic approaches for proteins and nucleic acids. Of equal importance to
  the synthesis of the biopolymers\, however\, are methods for their sequen
 cing.  Revolutions in nucleic acid sequencing have led to single molecule
  and Next-Gen parallel methods.  Similar advances in protein sequencing h
 ave lagged behind. In collaboration with the Marcotte group at UT Austin\,
  we have created a single-molecule peptide sequencing routine referred to 
 as fluorosequencing. Therein\, peptides are N-terminal captured\, the amin
 o acids selectively labelled with fluorophores\, C-terminal differentiated
 \, and then placed on TIRF microscope for rounds of Edman degradation. The
  development and implementation of the organic chemistry necessary in the 
 method will be discussed. On a related topic\, the sequencing of sequence-
 defined polymers\, other than nucleic acids and proteins\, shows promise a
 s a new paradigm for data storage.  We have devised the first use of olig
 ourethanes for storing and reading encoded information.  As a proof of pr
 inciple\, an approach will be described using a text passage from Jane Aus
 ten’s Mansfield Park. It was encoded in oligourethanes and reconstructed
  via chain-end degradation sequencing. We developed Mol.E-coder\, a softwa
 re tool that utilizes a Huffman encoding scheme to convert the character t
 able to hexadecimal. The passage was capable of being reproduced wholly in
 tact by a third-party\, without any purifications or the use of MS/MS\, de
 spite multiple rounds of compression\, encoding\, and synthesis. Further\,
  we have used mass-tags on the oligourethanes to sort mixtures and keep tr
 ack of simultaneous sequencing\, and we have recently generated electroche
 mical methods for sequencing. Overall\, this presentation will highlight t
 he interplay between synthesis and sequencing of sequence-defined polymers
 .
LOCATION:Dept of Chemistry\, Wolfson Lecture Theatre 
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