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SUMMARY:Fluorescence-Lifetime Super-Resolution Microscopy - Prof Jörg End
 erlein\, Georg August University\, Göttingen
DTSTART:20260224T140000Z
DTEND:20260224T150000Z
UID:TALK241243@talks.cam.ac.uk
CONTACT:David Madden
DESCRIPTION:Recent advancements in super-resolution microscopy have enable
 d unprecedented insights into the spatial organization of cellular structu
 res. In this talk\, I will present a series of methodological innovations 
 that synergistically integrate fluorescence-lifetime single-molecule local
 ization microscopy (FL-SMLM) [1\,2]\, image scanning microscopy (ISM) [3\,
 4]\, and metal-/graphene-induced energy transfer (MIET/GIET) imaging [5–
 7]. These approaches collectively offer isotropic three-dimensional resolu
 tion at the nanometer scale\, multiplexed imaging capabilities\, and robus
 tness against chromatic aberrations.\n\nFirst\, I will discuss our work on
  MIET and GIET microscopy\, which exploit distance-dependent quenching phe
 nomena near metallic or graphene interfaces to determine the axial positio
 n of single emitters with sub-10 nm accuracy. The combination of MIET with
  dSTORM or DNA-PAINT provides truly isotropic 3D resolution\, extending th
 e reach of localization microscopy into the axial dimension without interf
 erometric complexity.\n\nSecond\, I will highlight the development of fluo
 rescence lifetime DNA-PAINT (FL-PAINT)\, a technique that enables multi-ta
 rget super-resolution imaging through fluorescence lifetime multiplexing w
 ithout fluid exchange. By utilizing orthogonally designed imager strands c
 onjugated to fluorophores with distinct lifetimes\, we achieve simultaneou
 s imaging of multiple targets in the dense intracellular environment.\n\nL
 astly\, I will introduce our latest development of fluorescence-lifetime i
 mage scanning microscopy SMLM (FL-iSMLM)\, which achieves a near twofold e
 nhancement in lateral resolution by integrating a single-photon detector a
 rray into a confocal laser scanning microscope. This method combines the l
 ocalization precision of ISM with the multiplexing power of fluorescence-l
 ifetime detection\, enabling sub-5 nm resolution in fixed cells while simu
 ltaneously allowing discrimination of targets based solely on their fluore
 scence lifetimes.\n\nReferences\n\n[1] J. C. Thiele\, D. A. Helmerich\, N.
  Oleksiievets\, R. Tsukanov\, E. Butkevich\, M. Sauer\, O. Nevskyi\, and J
 . Enderlein\, Confocal Fluorescence-Lifetime Single-Molecule Localization 
 Microscopy\, ACS Nano 14\, 14190 (2020).\n\n[2] J. C. Thiele\, O. Nevskyi\
 , D. A. Helmerich\, M. Sauer\, and J. Enderlein\, Advanced Data Analysis f
 or Fluorescence-Lifetime Single-Molecule Localization Microscopy\, Front. 
 Bioinform. 1\, 740281 (2021).\n\n[3] C. B. Müller and J. Enderlein\, Imag
 e Scanning Microscopy\, Phys. Rev. Lett. 104\, 198101 (2010).\n\n[4] N. Ra
 dmacher\, O. Nevskyi\, J. I. Gallea\, J. C. Thiele\, I. Gregor\, S. O. Riz
 zoli\, and J. Enderlein\, Doubling the resolution of fluorescence-lifetime
  single-molecule localization microscopy with image scanning microscopy\, 
 Nat. Photon. 18\, 1059 (2024).\n\n[5] A. I. Chizhik\, J. Rother\, I. Grego
 r\, A. Janshoff\, and J. Enderlein\, Metal-induced energy transfer for liv
 e cell nanoscopy\, Nature Photon 8\, 124 (2014).\n\n[6] A. Ghosh\, A. Shar
 ma\, A. I. Chizhik\, S. Isbaner\, D. Ruhlandt\, R. Tsukanov\, I. Gregor\, 
 N. Karedla\, and J. Enderlein\, Graphene-based metal-induced energy transf
 er for sub-nanometre optical localization\, Nat. Photonics 13\, 860 (2019)
 .\n\n[7] J. C. Thiele\, M. Jungblut\, D. A. Helmerich\, R. Tsukanov\, A. C
 hizhik\, A. I. Chizhik\, M. Schnermann\, M. Sauer\, O. Nevskyi\, and J. En
 derlein\, Isotropic Three-Dimensional Dual-Color Super-Resolution Microsco
 py with Metal-Induced Energy Transfer\, Science Advances 8\, 14190 (2021).
LOCATION:Unilever Lecture Theatre\, Department of Chemistry
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