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SUMMARY:In vivo quantitative proteomics to study skin cancer progression -
  Sara Zanivan\, Department of Proteomics and Signal Transduction\, Max-Pla
 nck Institute for Biochemistry
DTSTART:20100422T120000Z
DTEND:20100422T130000Z
UID:TALK24176@talks.cam.ac.uk
CONTACT:Katrien Van Look
DESCRIPTION:A two-stage skin carcinogenesis mouse model\, the SILAC mouse1
  and high-resolution mass spectrometry were combined to investigate defect
 s in regulatory circuits in cutaneous carcinoma. In depth analysis of the 
 proteome and phosphoproteome of normal skin\, TPA-treated skin\, papilloma
  and squamous cell carcinoma was performed. This approach led to the ident
 ification of more than 6\,000 proteins and 13\,000 phosphorylation sites. 
 Quantitative analysis revealed regulation of proteins known to play a role
  in carcinoma development. As expected\, protein substrates for MAPKs and 
 CDKs were highly phosphorylated\, providing a positive control\, but the v
 ast majority of phosphorylation changes are novel. We conclude that our qu
 antitative in vivo (phospho) proteomic approach is a powerful strategy to 
 discover new molecules in carcinoma development for further elucidation.\n
 \n1Krüger M\, Moser M\, Ussar S\, Thievessen I\, Luber CA\, Forner F\, Sc
 hmidt S\, Zanivan S\, Fässler R\, Mann M. Cell. 2008 Jul 25\;134(2):353-6
 4.\n
LOCATION:Cancer Research UK Cambridge Research Institute\, Lecture Theatre
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