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SUMMARY:Noncoding human Y RNAs are overexpressed in tumours and required f
 or cell proliferation - Stefan Graf
DTSTART:20100426T143000Z
DTEND:20100426T153000Z
UID:TALK24659@talks.cam.ac.uk
CONTACT:Stefan Gräf
DESCRIPTION:Christov CP\, Trivier E\, Krude T.\nBr J Cancer. 2008 Mar 11\;
 98(5):981-8. Epub 2008 Feb 19.\nhttp://www.ncbi.nlm.nih.gov/pubmed/1828331
 8\n\n*Abstract*\nNoncoding Y RNAs have recently been identified as essenti
 al factors for chromosomal DNA replication in human cell nuclei. Here\, we
  investigate the expression of human Y RNAs in tumours and test their requ
 irement for cell proliferation. Relative expression levels of all four hum
 an Y RNAs (hY1\, hY3\, hY4 and hY5 RNA) were determined by quantitative RT
 -PCR in extracts from human solid tumours\, corresponding nonmalignant nor
 mal tissues and derived cultured cells. On average\, all four hY RNAs are 
 significantly overexpressed in solid tumours between 4- and 13-fold\, comp
 ared to the corresponding normal tissues. In particular\, hY1 and hY3 RNAs
  are overexpressed in carcinomas (and adenocarcinomas) of the bladder\, ce
 rvix\, colon\, kidney\, lung and prostate with extremely high statistical 
 significance (ANOVA\, between groups\, P<10e-22). A functional requirement
  of all four hY RNAs for cell proliferation was investigated in a systemat
 ic survey for loss-of-function by RNA interference (RNAi). Degradation of 
 hY1 and hY3 RNAs in human cell lines resulted in a significant cytostatic 
 inhibition of cell proliferation. We conclude that noncoding hY RNAs have 
 potential both as new cancer biomarkers and as molecular targets for anti-
 proliferative intervention.\n
LOCATION:Room 132\, CRI
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