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SUMMARY:Mapping Synthetic Genetic Interaction Networks by High-throughput 
 RNAi - Michael Boutros\, Division of Signaling and Functional Genomics\, G
 erman Cancer Research Center
DTSTART:20100527T120000Z
DTEND:20100527T130000Z
UID:TALK24958@talks.cam.ac.uk
CONTACT:Katrien Van Look
DESCRIPTION:To uncover the causal relationship between phenotypes and the 
 underlying genotypes is a long-standing challenge in biology. Identificati
 on of quantitative trait loci in crosses of yeast strains shows evidence t
 hat many heritable traits have interacting loci\, highlighting the role of
  genetic interactions in the pathogenesis of complex diseases. Synthetic g
 enetic interaction analysis can be used to study the functional relationsh
 ips between genes on a genome-wide scale\, yielding genetic interaction pr
 ofiles that sensitively reflect their molecular functions. We have develop
 ed a robust and scalable framework to quantitatively measure genetic inter
 action profiles in Drosophila cells\, using multiparametric cellular imagi
 ng and pairwise RNA interference.  Mathematical modelling of the double pe
 rturbations allow to reconstruct known molecular pathways. A second topic 
 of the talk will be the analysis of signaling pathways using high-dimensio
 nal phenotyping and large-scale perturbation studies.
LOCATION:Cancer Research UK Cambridge Research Institute\, Lecture Theatre
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