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SUMMARY:Human RNA polymerase III transcriptomes and relationships to Pol I
 I promoter chromatin and enhancer-binding factors. - Claudia Kutter (Cance
 r Research UK\, Cambridge Research Institute)
DTSTART:20100628T143000Z
DTEND:20100628T153000Z
UID:TALK25355@talks.cam.ac.uk
CONTACT:Stefan Gräf
DESCRIPTION:Nat Struct Mol Biol.  2010 May\;17(5):620-8. Epub 2010 Apr 25.
  Pubmed: "20418882":http://www.ncbi.nlm.nih.gov/pubmed/20418882\n\nAbstrac
 t\n\nRNA polymerase (Pol) III transcribes many noncoding RNAs (for example
 \, transfer RNAs) important for translational capacity and other functions
 . We localized Pol III\, alternative TFIIIB complexes (BRF1 or BRF2) and T
 FIIIC in HeLa cells to determine the Pol III transcriptome\, define gene c
 lasses and reveal 'TFIIIC-only' sites. Pol III localization in other trans
 formed and primary cell lines reveals previously uncharacterized and cell 
 type-specific Pol III loci as well as one microRNA. Notably\, only a fract
 ion of the in silico-predicted Pol III loci are occupied. Many occupied Po
 l III genes reside within an annotated Pol II promoter. Outside of Pol II 
 promoters\, occupied Pol III genes overlap with enhancer-like chromatin an
 d enhancer-binding proteins such as ETS1 and STAT1. Moreover\, Pol III occ
 upancy scales with the levels of nearby Pol II\, active chromatin and CpG 
 content. These results suggest that active chromatin gates Pol III accessi
 bility to the genome.
LOCATION:Room 132\, CRI
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