BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Binding Site Turnover Produces Pervasive Quantitative Changes in T
 ranscription Factor Binding between Closely Related Drosophila Species - K
 lara Stefflova (Cancer Research UK\, Cambridge Research Institute)
DTSTART:20100726T143000Z
DTEND:20100726T153000Z
UID:TALK25391@talks.cam.ac.uk
CONTACT:Stefan Gräf
DESCRIPTION:Bradley RK\, Li XY\, Trapnell C\, Davidson S\, Pachter L\, Chu
  HC\, Tonkin LA\, Biggin MD\, Eisen MB.\n\nPLoS Biol. 2010 Mar 23\;8(3):e1
 000343.\n[ "Pubmed":http://www.ncbi.nlm.nih.gov/pubmed/20351773 ]\n\nChang
 es in gene expression play an important role in evolution\, yet the molecu
 lar mechanisms underlying regulatory evolution are poorly understood. Here
  we compare genome-wide binding of the six transcription factors that init
 iate segmentation along the anterior-posterior axis in embryos of two clos
 ely related species: Drosophila melanogaster and Drosophila yakuba. Where 
 we observe binding by a factor in one species\, we almost always observe b
 inding by that factor to the orthologous sequence in the other species. Le
 vels of binding\, however\, vary considerably. The magnitude and direction
  of the interspecies differences in binding levels of all six factors are 
 strongly correlated\, suggesting a role for chromatin or other factor-inde
 pendent forces in mediating the divergence of transcription factor binding
 . Nonetheless\, factor-specific quantitative variation in binding is commo
 n\, and we show that it is driven to a large extent by the gain and loss o
 f cognate recognition sequences for the given factor. We find only a weak 
 correlation between binding variation and regulatory function. These data 
 provide the first genome-wide picture of how modest levels of sequence div
 ergence between highly morphologically similar species affect a system of 
 coordinately acting transcription factors during animal development\, and 
 highlight the dominant role of quantitative variation in transcription fac
 tor binding over short evolutionary distances.
LOCATION:Room 132\, CRI
END:VEVENT
END:VCALENDAR