BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Molecular organization and signaling at the T-cell surface: system
 -level and single molecule-based analysis - Professor Simon Davis\, Univer
 sity of Oxford
DTSTART:20101020T113000Z
DTEND:20101020T123000Z
UID:TALK26161@talks.cam.ac.uk
CONTACT:Sue Griffin
DESCRIPTION:In the past we identified what turned out to be the complete s
 et of T cell-specific proteins expressed at the resting T-cell surface and
  showed how\, for key examples\, they interact with their ligands. We also
  proposed\, with P.A. van der Merwe\, a counter-intuitive explanation for 
 how key receptors are “triggered” by their ligands\, now known as the 
 “kinetic-segregation” model.\n\nOur present research is extending thes
 e analyses of the functions and behaviour of leukocyte receptors. We have 
 an experimental framework for testing the kinetic-segregation model at the
  level at which it is proposed to operate\, i.e. the single-molecule level
 . We have also established methods for the quantitative treatment of biolu
 minescence resonance energy transfer data that we are using to establish t
 he organizational “ground-state” of the T-cell surface. We also plan t
 o reconstitute the triggering apparatus of the T cell in insect cells in o
 rder to identify the minimum set of molecules required for triggering. \n\
 nWe have undertaken the very deep analysis of the CD4+ T-cell transcriptom
 e and are using this for whole-cell level simulations of the earliest post
 -triggering event in signal transduction\, i.e. the recruitment of SH2 dom
 ains to triggered\, tyrosine-phosphorylated receptors. Finally\, we are ap
 plying our understanding of the triggering process to the development of i
 nhibitory antibody superagonists as a potential general strategy for the t
 reatment of common autoimmune diseases. We hope to obtain proof-of-concept
  for the therapeutic principle following the generation of a novel “knoc
 k-in” mouse model of human autoimmunity.\n
LOCATION:Lecture Theatre\, Department of Pathology\, Tennis Court Road
END:VEVENT
END:VCALENDAR