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SUMMARY:Specificity and function of NK cells: interplay between HLA class 
 I and KIR gene polymorphism - Dr Markus Uhrberg\, Heinrich Heine Universit
 y\, Dusseldorf
DTSTART:20101201T123000Z
DTEND:20101201T133000Z
UID:TALK26909@talks.cam.ac.uk
CONTACT:Sue Griffin
DESCRIPTION:Dr. Uhrberg is working in the field of natural killer cell bio
 logy. He was the first to describe the polymorphism of the KIR gene family
  and to develop DNA- as well as RNA-based KIR typing protocols(1). Later o
 n\, he discovered the epigenetic regulation of KIR gene expression by DNA 
 methylation and histone modifications as well as the reactivation of silen
 ced KIR genes by epigenetic drugs(2\, 3). Dr. Uhrberg runs an internationa
 lly accredited immunogenetics facility (ASHI-approved SOP for KIR typing) 
 and is reference laboratory of the International KIR exchange program orga
 nized by UCLA since 2004. Recently\, the group developed multiparameter fl
 ow cytometry techniques to analyze the functional NK cell repertoire on th
 e clonal level\, and in combination with either markers for degranulation 
 (CD107)\, cytokine production (IFNy)\, or components of the lytic machiner
 y (granzyme B\, perforin) to assess the mechanisms that lead to structural
  and functional education of NK cell repertoires(4)\n\nReferences\n\n1.	Uh
 rberg\, M.\, N. M. Valiante\, B. P. Shum\, H. G. Shilling\, K. Lienert-Wei
 denbach\, B. Corliss\, D. Tyan\, L. L. Lanier\, and P. Parham. 1997. Human
  diversity in killer cell inhibitory receptor genes. Immunity 7:753-763.\n
 2.	Santourlidis\, S.\, H. I. Trompeter\, S. Weinhold\, B. Eisermann\, K. L
 . Meyer\, P. Wernet\, and M. Uhrberg. 2002. Crucial role of DNA methylatio
 n in determination of clonally distributed killer cell Ig-like receptor ex
 pression patterns in NK cells. J Immunol 169:4253-4261.\n3.	Santourlidis\,
  S.\, N. Graffmann\, J. Christ\, and M. Uhrberg. 2008. Lineage-specific tr
 ansition of histone signatures in the killer cell Ig-like receptor locus f
 rom hematopoietic progenitor to NK cells. J Immunol 180:418-425.\n4.	Schö
 nberg\, K.\, M. Sribar\, J. Enczmann\, J. C. Fischer\, and M. Uhrberg. Ana
 lyses of HLA-C-specific KIR repertoires in donors with group A and B haplo
 types suggest a ligand-instructed model of NK cell receptor aquisition. Bl
 ood\, in press.\n\n\n
LOCATION:Lecture Theatre\, Department of Pathology\, Tennis Court Road
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