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SUMMARY:Decoding Genetic Switches in T Helper Cell Differentiation - Sarah
  Teichmann (LMB)
DTSTART:20101110T140000Z
DTEND:20101110T150000Z
UID:TALK27393@talks.cam.ac.uk
CONTACT:Danielle Stretch
DESCRIPTION:Gene expression levels are believed to be continuously distrib
 uted from very low to very high levels\, with most genes at an intermediat
 e level.  We have studied the transcriptome-wide distribution of expressio
 n levels in mouse T helper cells using RNA-seq technology\, and have devel
 oped a variety of ways to model the expected background levels.  This is c
 ritical for definition of a threshold level of expression of a gene in a g
 iven cell type.  In order to calibrate the RNA-seq expression levels in te
 rms of molecules of mRNA per cell\, we have intregrated the RNA-seq data w
 ith single molecule mRNA-FISH experiments.\n\nThe results of these analyse
 s and experiments show that many genes are expressed at > ~1 molecule per 
 cell and that two major expression levels can be identified which vary by 
 roughly one to two orders of magnitude.  This gives rise to bimodal distri
 butions of gene expression levels in cell populations.  Analysis of histon
 e modifications by ChIP-seq indicates that activating modifications such a
 s H3K9/14ac and H3K4me3 are involved in this "digital" expression switch.\
 n\nOut findings have broad implications for the analysis RNA-seq and ChIP-
 seq data and for the understanding of the regulation of gene expression.
LOCATION:MR4\, Centre for Mathematical Sciences\, Wilberforce Road\, Cambr
 idge
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