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SUMMARY:Recombinatorial cloning tools for probing Staphylococcus aureus - 
 Dr Phil Hill\, Associate Professor of Microbiology\, Faculty of Science\, 
 Nottingham
DTSTART:20110302T163000Z
DTEND:20110302T173000Z
UID:TALK28480@talks.cam.ac.uk
CONTACT:Suzy Blows
DESCRIPTION:Many bacterial pathogens are problematic to analyse due to a p
 aucity of molecular tools and intrinsic properties of the organisms such a
 s poor transformation efficiencies or low recombination frequencies. We ha
 ve engineered vectors compatible with recombinatorial cloning for plasmid 
 and chromosomally integrated expression and validated them in a number of 
 organisms using reporter gene constructs. A mutagenesis system combining r
 ecombinatorial cloning with marker excision\, mediated by ΦC31 integrase\
 , to make sequential knockout mutants in Gram positive pathogens has facil
 itated the study of the intracellular lifestyle of Staphylococcus aureus.
LOCATION:Lecture Theatre 1\, Department of Veterinary Medicine
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