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SUMMARY:A quantitative view of gene expression levels in T helper cells - 
 Dr Sarah Teichmann\, MRC Programme Leader\, Computational Genomics\, MRC L
 aboratory of Molecular Biology hosted by Doug Fearon
DTSTART:20110114T130000Z
DTEND:20110114T140000Z
UID:TALK28624@talks.cam.ac.uk
CONTACT:Mia Summerfield
DESCRIPTION:Gene expression levels are believed to be continuously distrib
 uted from very low to very high levels\, with most genes at an intermediat
 e level. We have studied the transcriptome-wide distribution of expression
  levels in mouse T helper cells using RNA-seq technology\, and have develo
 ped a variety of ways to model the expected background levels. This is cri
 tical for definition of a threshold level of expression of a gene in a giv
 en cell type. In order to calibrate the RNA-seq expression levels in terms
  of molecules of mRNA per cell\, we have integrated the RNA-seq data with 
 single molecule mRNA-FISH experiments. \nThe results of these analyses and
  experiments show that many genes are expressed at > ~ 1 molecule per cell
  and that two major expression levels can be identified which vary by roug
 hly one to two orders of magnitude. This gives rise to bimodal distributio
 ns of gene expression levels in cell populations. Analysis of histone modi
 fications by ChIP-seq indicates that activating modifications such as H3K9
 /14ac and H3K4me3 are involved in this ‘digital’ expression switch. \n
 Our findings have broad implications for the analysis RNA-seq and ChIP-seq
  data and for the understanding of the regulation of gene expression.\n
LOCATION:Sackler Lecture Theatre (Level 7)\, Cambridge Institute for Medic
 al Research
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