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SUMMARY:Quantitative Cell Imaging with Digital Holographic Microscopy - Bj
 örn Kemper\, University of Münster\, Germany
DTSTART:20110209T101500Z
DTEND:20110209T111500Z
UID:TALK29756@talks.cam.ac.uk
CONTACT:Dr. Jochen Guck
DESCRIPTION:Digital holographic microscopy (DHM) provides contact-less\, l
 abel-free\, quantitative phase imaging for modular integration into commer
 cial microscopes. The reconstruction of digitally captured holograms is pe
 rformed numerically. Thus\, multi-focus imaging of sample parts in differe
 nt layers is achieved from a single digital hologram.\n\nThe evaluation of
  the obtained quantitative phase contrast images provides data for dynamic
  thickness monitoring\, and the analysis of fluidics. The DHM feature of (
 subsequent) numerical autofocus enables applications in the field of live 
 cell analysis by avoiding mechanical focus realignment and the determinati
 on of axial sample positions. In this way\, long term time-lapse investiga
 tions in toxicology and cancer research\, monitoring of fast dynamic proce
 sses like shape variations and 3D cell tracking is achieved. In addition\,
  the integral cellular refractive index and its statistics are available.\
 n\nIn an overview results from investigations with different DHM arrangeme
 nts on toxin induced reactions of cancer cells\, cell swelling kinetics\, 
 cell division analysis as well as refractive index measurements are shown.
  Furthermore\, the analysis of the cell response to optical manipulation\,
  shape variations of sedimenting human erythrocytes\, reactions of cells t
 o different substrates and 3D cancer cell migration in a 3D tissue model p
 rospect new application fields of DHM for quantitative cell imaging in Lif
 e Sciences.\n\n
LOCATION:Physics of Medicine Seminar Room\, PoM Building\, Cavendish Labor
 atory\, Department of Physics
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