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SUMMARY:Discovery of sRNA-mediated gene regulation in Salmonella - Profess
 or Jay Hinton\, Department of Microbiology\, Trinity College\, Dublin
DTSTART:20110518T153000Z
DTEND:20110518T163000Z
UID:TALK30579@talks.cam.ac.uk
CONTACT:Suzy Blows
DESCRIPTION:Salmonella enterica serovar Typhimurium is an adaptable and ro
 bust micro-organism that thrives in a variety of environmental niches\, in
 cluding the GI tract of humans\, farm animals\, birds and reptiles\, and i
 nside mammalian cells. To succeed as a pathogen\, Salmonella relies upon a
  combination of regulatory proteins and two component systems to adapt the
  bacteria to every stage of infection. It has now become clear that small 
 RNA molecules (sRNAs) mediate a relatively new type of gene regulation\, a
 nd control the virulence of Listeria\, Staphylococcus\, Streptococcus and 
 Vibrio. If we are to understand the aspects of Salmonella gene expression 
 that are critical for survival\, adaptation and disease\, the sRNA biology
  of this important model pathogen must be defined. \n\nWe used a combinati
 on of chromatin immunoprecipitation and deep sequencing to identify the sR
 NAs of S. Typhimurium that are associated with Hfq (Sittka et al.\, 2008 P
 LoS Genetics 4: e1000163)\, and more than 130 sRNAs have now been experime
 ntally verified in Salmonella. I will describe the role played by small re
 gulatory RNA (sRNA) molecules in the rapid targeting and removal of mRNAs 
 from the bacterial cell. The role of sRNA-mediated control of bacterial st
 ress responses in transcriptional networks will be discussed.\n\nWe are cu
 rrently identifying and auditing the sRNAs of S. Typhimurium expressed und
 er multiple environmental conditions\, including the infection process. Ou
 r comprehensive deep sequencing-based RNomic analysis has already identifi
 ed hundreds of new Salmonella sRNAs. We have experimental evidence for 9 n
 ovel Salmonella-specific sRNAs that are expressed in a variety of environm
 ents. These include conditions that induce expression of Salmonella pathog
 enicity islands SPI1 and SPI2\, and within murine macrophages.\n
LOCATION:Lecture Theatre 1\, Department of Veterinary Medicine
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