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SUMMARY:Identification &amp\; characterization of CML23 and CML24 in Arabi
 dopsis thaliana circadian clock - Nur Izzati Mohd Noh
DTSTART:20110603T123000Z
DTEND:20110603T130000Z
UID:TALK30695@talks.cam.ac.uk
CONTACT:Suzy Stoodley
DESCRIPTION:The circadian clock is important to all organisms as an intern
 al time-keeper\, which facilitates adaptations to the external environment
  conditions. In plants\, several biological mechanisms such as leaf moveme
 nt and cytosolic Ca2+ concentration are regulated by the circadian clock. 
 In this study\, I will identify the function of CML23 and CML24\, calmodul
 in-like proteins\, in the Arabidopsis thaliana circadian clock. There are 
 50 CML genes in A. thaliana\, encoding potential Ca2+ sensors. cml23-2cml2
 4-4 double mutants lengthened the period of circadian leaf movement rhythm
 s compared to wild type (Col-0)\, although cml23-2ml24-1 double mutants ha
 d unaltered leaf movement rhythms. These results suggested that CML23 and 
 CML24 may have overlapping roles in regulation of the Arabidopsis central 
 circadian oscillator. Database queries from www.asociomics.com have result
 ed in identification of seven putative interacting partners of CML24. Howe
 ver\, no results were obtained for CML23 from the same database. CML24 mig
 ht interact with these putative interacting partners to indirectly regulat
 e the intracellular processes. Protein pull down analysis will be performe
 d to identify any potential interactions between CML24 with its interactin
 g partners. The circadian leaf movement rhythm analysis of cpk3\, cpk6 and
  cpk3cpk6 had show effect in term of phase shift in cpk3-1 single mutant a
 nd cpk3-1cpk6-1 double mutants\, but not the period. Although the data are
  preliminary\, these results suggest a hypothesis that CPK3 acts downstrea
 m of CML24 to control the circadian clock of A. thanliana In addition\, ep
 istatic analysis will be carried out to outline the potential points of en
 try for CML23 and CML24 in the circadian system. To address the epistatic 
 relationship\, cml23-2cml24-4 double mutants will be crossed with known cl
 ock loss of function mutants such as toc1-1\, ztl1\, abi1-1 lux\, elf3-1\,
  elf4-1\, lhy and cca1-1. The circadian leaf movement rhythm analysis will
  be used to screen for the phenotype of the triple mutants. At the end of 
 this study\, these findings will direct future understanding of the role o
 f CML23 and CML24 in the circadian clock regulation. 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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