BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Structural and Mechanistic Studies of the Anaphase Promoting Compl
 ex - David Barford\, Institute of Cancer Research
DTSTART:20111124T161500Z
DTEND:20111124T180000Z
UID:TALK31588@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:The anaphase-promoting complex (APC) is an unusually large E3 
 ubiquitin ligase that controls defined cell cycle transitions such as the 
 metaphase to anaphase transition and the exit from mitosis. Its activity a
 nd substrate specificity are regulated in a cell cycle-dependent manner by
  co-activator subunits\, inhibitory protein complexes and reversible prote
 in phosphorylation. To understand the molecular basis of APC function we h
 ave undertaken a structural analysis of different functional states of the
  complex through a combination of X-ray crystallography and single particl
 e electron microscopy. Generation of a recombinant over-expression system 
 that allowed reconstitution of the intact complex facilitated identificati
 on of individual APC subunits within the EM-derived molecular envelope\, l
 eading to the generation of pseudo-atomic models. I will describe our expe
 rimental approaches and how the resultant structures provide insights into
  APC architecture and mechanisms.
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
END:VEVENT
END:VCALENDAR