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SUMMARY:RNAi libraries for high throughput phenotyping and drug resistance
  screening in the African trypanosome - David Horn (LSHTM)
DTSTART:20111205T160000Z
DTEND:20111205T170000Z
UID:TALK31871@talks.cam.ac.uk
CONTACT:Florian Markowetz
DESCRIPTION:African trypanosomes are major pathogens of humans and livesto
 ck and represent a model for studies of unusual protozoal biology. We deve
 loped a high throughput phenotyping approach termed RNA interference (RNAi
 ) target sequencing\, or RIT-seq that\, using Illumina sequencing\, maps f
 itness-costs associated with RNAi. Analysis of bloodstream and insect life
 -cycle stages and differentiated libraries linked thousands of previously 
 uncharacterised and ‘hypothetical’ genes to essential functions. The d
 ata-set also provides much needed genetic validation to identify new drug-
 targets. In a second application\, all five current anti-trypanosomal drug
 s were used for RIT-seq screens\, revealing the transporters\, organelles\
 , enzymes and metabolic pathways that function to facilitate anti-trypanos
 omal drug action.
LOCATION:Cancer Research UK Cambridge Research Institute\, Room 215
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