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SUMMARY:New Methods of Bioanalysis using Functionalised Nanoparticles and 
 SERS - Professor Duncan Graham (University of Strathclyde ) http://www.che
 m.strath.ac.uk/people/academic/duncan_graham
DTSTART:20111128T143000Z
DTEND:20111128T160000Z
UID:TALK33590@talks.cam.ac.uk
CONTACT:Paula Charnock
DESCRIPTION:Functionalized nanoparticles have been used in a number of dif
 ferent studies\, including detection of DNA at ultra low levels\, immuno h
 istochemistry and\, more recently\, as substrates for surface enhanced res
 onance Raman scattering (SERRS) based imaging approaches.  The advantages 
 of using metallic nanoparticles are that they are very bright in terms of 
 their optical characteristics and also\, if functionalized in a particular
  manner\, provide a SERRS response to give a unique vibrational fingerprin
 t.  Here we present the functionalization of gold and silver nanoparticles
  in such a way that the enhancement effect can be greatly increased throug
 h biological recognition and\, as such\, this effectively turns on the SER
 RS effect.  This process can give rise to exquisite selectivity in terms o
 f the interaction of the nanoparticles\, especially when DNA hybridization
 s are used\, and single base mismatches can be analyzed at room temperatur
 e.  Dye oligonucleotide silver nanoparticles (DOSN) have also been used to
  detect double-stranded DNA through triplex formation to switch on the SER
 RS and a distance relationship between nanoparticles and SERRS response ha
 s been established for the first time.  In an advancement of this approach
 \, functionalized nanoparticles have also been used as imaging agents for 
 single cells and\, when functionalized with an appropriate antibody\, can 
 give back information on the expression of specific receptors on cell surf
 aces\, as well as sub-cellular compartmentalization information.  Finally\
 , in moving away from the in vitro applications\, the functionalized nanop
 articles can be modified in such a way that they are active in vivo and pr
 eliminary data relating to in vivo studies of imaging and therapeutic uses
  of functionalized SERRS active nanoparticles will also be presented.  Thi
 s presentation covers the full range of design\, the optical properties\, 
 and finally the biological properties of functionalized nanoparticles in r
 elation to specific disease states. 
LOCATION:Todd Hamied Room\,  Department of Chemistry
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