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SUMMARY:From protein interactions to gene expression distributions - Sarah
  Teichmann (LMB)
DTSTART:20120306T160000Z
DTEND:20120306T170000Z
UID:TALK34376@talks.cam.ac.uk
CONTACT:Aleksandra Watson
DESCRIPTION:From protein interactions to gene expression distributions\n\n
 The work in my group is focused on the evolution and dynamics of protein i
 nteractions and transcriptional regulatory interactions. In these two area
 s\, we analyse large datasets using computational and mathematical methods
 .\n\nIn the first part of my presentation\, I will talk about our work on 
 the evolution and assembly of protein complexes. We surveyed three-dimensi
 onal structures of protein complexes to identify interface size as a deter
 mining principle of both evolutionary and assembly pathways (Levy et al.\,
  Nature\, 2008)\, and to quantify conformational change in assembly (Marsh
  & Teichmann\, Structure\, 2011). In recent work\, we show for the first t
 ime\, the importance of promiscuous protein interactions in determining a 
 protein’s surface residue composition (Levy et al.\, in preparation). Th
 ese new insights have implications for structure prediction and protein en
 gineering. \n\nIn the second part of my presentation\, I will talk about o
 ur work on dissecting the distribution of gene expression levels in mammal
 ian cell populations\, revealing two distinct mRNA abundance classes (Hebe
 nstreit et al.\, Mol Sys Biol\, 2011). We provide evidence\, including cor
 relation of the two mRNA abundance classes with epigenetic modifications (
 Hebenstreit et al.\, Nucleic Acids Res\, 2011)\, supporting the notion tha
 t these two classes correspond to functional versus non-functional protein
 s in cells. These findings now help interpret mRNA and protein abundance d
 ata in the form of microarrays\, RNA-sequencing and proteomics.\n\nReferen
 ces \n\nLevy\, E.D.\, Boeri-Erba\, E.\, Robinson\, C.V. & Teichmann\, S.A.
  (2008) Assembly reflects evolution of protein complexes. Nature\, 453\, 1
 262-5.\n\nMarsh\, J. & Teichmann\, S.A. (2011) Relative solvent accessible
  surface area predicts protein conformational changes upon binding. Struct
 ure\, 19(6):859-67.\n\nHebenstreit\, D.\, Fang\, M.\, Gu\, M. Charoensawan
 \, V.\, van Oudenaarden\, A. & Teichmann\, S.A. (2011a) RNA sequencing rev
 eals two major classes of gene expression levels in metazoan cells. Mol. S
 ys. Biol.\, 7:497.\n\nHebenstreit\, D.\, Gu\, M.\, Haider\, S.\, Turner\, 
 D.\, Lio\, P. & Teichmann S.A. (2011b) EpiChIP: gene-by-gene quantificatio
 n of epigenetic modification levels. Nucleic Acids Res.\, 39\, e27.\n\n\n
LOCATION:Biochemistry Lecture Theatre\, Sanger Building
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