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SUMMARY:Functionalised nanoparticles and SERS for bioanalysis - Professor 
 Duncan Graham\,  University of Strathclyde\, UK
DTSTART:20120203T140000Z
DTEND:20120203T150000Z
UID:TALK35698@talks.cam.ac.uk
CONTACT:Tracy Inman
DESCRIPTION:Gold and silver nanoparticles can be functionalized in such a 
 way that the enhancement of Raman scattering can be greatly increased thro
 ugh biological recognition.  This process can give rise to exquisite selec
 tivity in terms of the interaction of the nanoparticles\, especially when 
 DNA hybridizations are used and single base mismatches can be analyzed at 
 room temperature.  Initially DNA-DNA based systems have been investigated 
 but updates on the ability to enhance SERS through nanoparticle assembly u
 sing DNA-protein and peptide-protein systems will be given.  In an alterna
 tive strategy to harness the power of SERS\, dyes which normally have a hi
 gh affinity for the metal used for enhancement can be masked to prevent th
 em adsorbing.  Exposure to a specific enzyme cleaves the mask and allows t
 he dye to adsorb generating a specific SERS signal which can be used to me
 asure enzyme activity and selectivity both in assay formats and in single 
 cells. 
LOCATION:Pippard Lecture Theatre\, Cavendish Laboratory
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