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SUMMARY:Using a new model to understand C4 gene regulation - Christopher J
 ohn
DTSTART:20120608T120000Z
DTEND:20120608T123000Z
UID:TALK36373@talks.cam.ac.uk
CONTACT:Suzy Stoodley
DESCRIPTION:C4 photosynthesis is a suite of biochemical\, ultrastructural\
 , and anatomical modifications that serve to concentrate CO2 around the en
 zyme RuBisCO. These modifications are typically associated with the partit
 ioning of photosynthetic reactions between mesophyll and bundle sheath cel
 ls. Currently while a significant amount of fundamental C4 research is bei
 ng carried out using dicot models\, there is a humanitarian incentive to a
 pply our understanding in monocots. For this reason and to expand upon bas
 ic scientific understanding it has been suggested Setaria viridis is utili
 sed as a new monocot model of C4 photosynthesis. Since maize is currently 
 the best studied C4 monocot I intend to extend our understanding of maize 
 promoters by exploring how they perform in Setaria. Simultaneously I inten
 d on examining the orthologus Setaria promoters to enable a comparison. To
  study cis elements I have established a vector system to place maize and 
 Setaria PEPC and PPDK promoter regions upstream of GUS\, and improved an i
 nfiltration protocol to transiently transform Setaria. To complement this 
 and study trans factors I have isolated two complexes in maize that bind t
 he PEPC promoter. In the future I intend to test whether protein complexes
  that bind Setaria promoters can be isolated. With these two aspects of my
  project I aim to use Setaria viridis as a new model to understand C4 gene
  regulation.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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