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SUMMARY:A motif present in multiple C4 genes is necessary for mesophyll sp
 ecificity - Ben Williams
DTSTART:20120517T150000Z
DTEND:20120517T153000Z
UID:TALK36389@talks.cam.ac.uk
CONTACT:Suzy Stoodley
DESCRIPTION:The C4 pathway has evolved in at least 66 lineages of angiospe
 rms. Typically\, the activity of enzymes associated with the core pathway 
 are restricted to either mesophyll (M) or bundle sheath (BS) cells\, and t
 he mechanisms generating this cell specificity are beginning to be underst
 ood for several genes. However\, there are very few examples in which the 
 same mechanism has been shown to underlie cell specificity of the multiple
  genes required for C4 photosynthesis. We recently showed that the untrans
 lated regions (UTRs) of both the pyruvate\,orthophosphate dikinase (PPDK) 
 and ß-carbonic anhydrase 4 (CA4) are sufficient to direct M-specificity i
 n C4 Cleome gynandra (Kajala et al 2012\, Plant J. 69\, 47–56). I now sh
 ow that the same mechanism is responsible for M-specific accumulation of P
 PDK and CA4 transcripts as well as CA2. Deletion analysis\, computational 
 prediction and site directed mutagenesis identified a 9-nucleotide motif i
 n both the 5' and 3' UTRs of PPDK and CA2/4 that is necessary for M-specif
 icity. In addition\, disruption of the secondary structure of UTRs also ab
 olishes M-specificity. A hypothesis for the regulation of these transcript
 s by UTR binding trans-acting factors at the translation initiation comple
 x is proposed. 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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