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SUMMARY:Chlamydomonas reinhardtii NADPH Oxidases as potential electron tra
 nsporters for biophotovoltaic devices. - Xander Anderson
DTSTART:20120621T153000Z
DTEND:20120621T160000Z
UID:TALK36687@talks.cam.ac.uk
CONTACT:Suzy Stoodley
DESCRIPTION:Biophotovoltaic devices can harvest electrons from the outer s
 urface (i.e plasma membrane) of algal cells (such as Chlamydomonas) and us
 e these to generate power. NADPH Oxidases (NOX) are plasma membrane enzyme
 s that transport electrons from intracellular NADPH to extracellular oxyge
 n\, generating superoxide anion. In other algae\, NOX are important for gr
 ow and toxicity\, and NOX are the primary electron transporters for biopho
 tovoltaic devices constructed with animal cells. C.reinhardtii harbours tw
 o putative NOX (RBOL1 and 2) which I am investigating as potential plasma 
 membrane electron transporter mechanisms. In the talk I will discuss NOX s
 equence analysis\, measurements of NOX activity and gene expression. I wil
 l also outline molecular techniques\, such as amiRNA silencing\, promoter 
 driven overexpression and GFP localisation\, which I am using to understan
 d both the biological function of these NOX in C.reinhardtii and the poten
 tial role they play as electron sources for biophotovoltaic devices.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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