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SUMMARY:Massively multi-parameter single cell data by Mass Cytometry:  the
  technology of its acquisition and networks for its interpretation - Scott
  Tanner\, University of Toronto and DVS Sciences
DTSTART:20120503T140000Z
DTEND:20120503T150000Z
UID:TALK37206@talks.cam.ac.uk
CONTACT:Laura Blackburn
DESCRIPTION:Mass Cytometry brings the power\, resolution\, sensitivity and
  quantitative\ncapabilities of atomic mass spectrometry to high throughput
  single cell\nanalysis in order to address the challenges of multi-paramet
 er\, quantitative\nflow cytometry.  Individual cells that have been immuno
 logically stained\nwith stable isotope tags are injected into the analytic
 al instrument that\n³reads² the tag elements.  The cells are vaporized\,
  atomized and ionized in\na high temperature plasma\, and the atomic compo
 sition of each cell \nincluding the metal tags  is measured by time of fli
 ght mass spectrometry.\nAdapted from its long-time use in elemental analys
 is\, the atomic mass\nspectrometer provides high sensitivity for many (up 
 to 100) independent mass\nchannels and offers the capability for absolute 
 quantification. At present\,\n35 stable isotopes of the metals are availab
 le as tags\, and we expect that\nanother 30 will be available in the fores
 eeable future with the eventual\npotential for 100.  The staining protocol
  is similar to that of flow\ncytometry\, and the data output is in FCS for
 mat for porting into third party\nflow cytometry analysis software.  Becau
 se the detection channels are\nindependent\, and the sensitivity to each p
 robe is similar\, the selection of\nstaining panels is trivial.  According
 ly\, it is as easy to quantitatively\nanalyze many parameters as a few\, f
 acilitated by the absence of need for\ncompensation.\n\nA high level intro
 ductory tutorial on the technology of element-labeling and\nanalysis will 
 be given. We will use data from our laboratory and that of our\ncollaborat
 ors in the Nolan group at Stanford University\, notably on\ndetermining di
 fferential immune and drug responses across a human\nhematopoietic continu
 um using 31 simultaneous cell surface and intracellular\nprobes\, to asses
 s the current art in multidimensional data analysis.\n\nScott is co-founde
 r and Chief Technology Officer (CTO) at DVS
LOCATION:Cancer Research UK Cambridge Research Institute\, Lecture Theatre
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