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SUMMARY:Simplification of topology during Xer-mediated recombination - Gra
 inge\, I (University of Newcastle\, Australia)
DTSTART:20120907T081000Z
DTEND:20120907T085000Z
UID:TALK39589@talks.cam.ac.uk
CONTACT:Mustapha Amrani
DESCRIPTION:The chromosome of the bacterium Escherichia coli is a single c
 ircular DNA molecule of about 4.6 Mbp. Various recombinational repair proc
 esses that act upon the DNA during or following its replication can lead t
 o a genetic crossover occurring\, which in turn leads to a chromosome dime
 r being formed. This requires resolution back to two un-catenated single c
 hromosomes before the cell can divide. The dimer resolution reaction is ca
 talysed by the two recombinase proteins\, XerC and XerD. Catalysis by XerD
  is in turn controlled by protein-protein interaction with the gamma subdo
 main of the FtsK DNA translocase. Recent in vitro data has shown that the 
 interaction between XerD and FtsK-gamma is sufficient to stimulate recombi
 nation\, but that in the absence of the DNA translocation motor of FtsK\, 
 then complex toplogical products result. If the motor is added back to the
 se reactions\, then simple\, unlinked circular products are seen. DNA tran
 slocation is required for efficient chromosome dimer resolution in vivo to
 o\, mirroring the other experimental results. The focus of current researc
 h is into how XerD catalytic activity is stimulated\, and how DNA transloc
 ation by FtsK leads to products with a simple toplogy. \n
LOCATION:Seminar Room 1\, Newton Institute
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