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SUMMARY:The substrate and function of mitochondrial ABC transporters invol
 ved in Fe-S metabolism  - Theresia Schaedler\, Plant Biochemistry
DTSTART:20121026T120000Z
DTEND:20121026T123000Z
UID:TALK40556@talks.cam.ac.uk
CONTACT:24938
DESCRIPTION:ABC transporters of the mitochondria (ATM) are required for cy
 tosolic Fe-S cluster assembly. They are located in the inner membrane of t
 he mitochondria and mediate ATP-driven transport of a so-far unknown compo
 und from the mitochondria into the cytosol. Previous studies in the model 
 plant Arabidopsis suggested that the transported compound contains sulphur
  (but not iron) in an unknown chemical form (1). Additionally\, a role of 
 GSH in yeast Atm1 function was shown (2).\n\nThe aim of our study is to ch
 aracterize the substrate specificity of the plant AtATM3 and its orthologu
 e yeast ScAtm1. We analyzed sulphur and thiol containing compounds in isol
 ated mitochondria using High Pressure Liquid Chromatography and observed c
 hanges of the ratios of GSH to other thiols in atm mutants in both yeast a
 nd Arabidopsis. Arabidopsis atm3 mutants are also sensitive to buthionine 
 sulfoximine (BSO)\, an inhibitor of the first step of glutathione synthesi
 s\, and as a result display reduced root growth. These data indicate that 
 GSH is important in ATM function - as previously suggested for yeast Atm1 
 (2).\n\nTo further investigate the substrate specificity of ATMs we have e
 stablished an expression and purification system for ATMs in the gram-posi
 tive bacterium Lactococcus lactis and are currently screening for potentia
 l substrates by monitoring the rate of ATP hydrolysis of purified potein. 
 Future work will include establishment of transport assays using the lacto
 coccal system.\n\n1. Bernard\, D. et al. (2009) Plant Physiol. 151: 590-60
 2\n\n2. Sipos\, K. et al. (2002) J. Biol. Chem. 277(30): 26944-26949 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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