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SUMMARY:Purine nucleoside phosphorylases in plants - Farhat Nazir\, Develo
 pmental Botany
DTSTART:20121130T133000Z
DTEND:20121130T140000Z
UID:TALK41765@talks.cam.ac.uk
CONTACT:24938
DESCRIPTION:Purine nucleoside phosphorylases (PNP) are enzymes thought to 
 be involved in purine metabolism and may have important roles in developme
 nt. PNPs are rarely reported in plants. A group of poplar proteins: bark s
 torage protein (BSP) and wound inducible (Win4) are the homologues of PNP 
 in plants. Dr Hanke’s lab has identified a protein\, cytokinin riboside 
 phosphorylase\, which is encoded by the gene locus At4g28940. In Arabidops
 is thaliana At4g24340 and At4g24350 are the only other genes encoding prot
 eins predicted to have purine riboside phosphorylase activity. They closel
 y resemble the BSP/Win4 homologues in poplar. In this study the full lengt
 h sequences of the two versions of At4g24340 and one of At4g24350 have bee
 n cloned in the pMal system and protein expressed for enzyme studies. The 
 system yielded a MBP::protein-of-interest fusion product which has been pu
 rified by affinity chromatography. The present aim is to characterise the 
 two predicted PNPs\, At4g24340 and At4g24350\, determining their enzyme ac
 tivity\, accumulation and the localisation of transcripts and proteins in 
 plant tissues and cell.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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