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SUMMARY:Direct regulation of physiological responses by the Arabidopsis ci
 rcadian clock component PRR7 - Eva Farre from Michigan State University
DTSTART:20121128T130000Z
DTEND:20121128T140000Z
UID:TALK41767@talks.cam.ac.uk
CONTACT:31151
DESCRIPTION:Up to 30 % of the plant transcriptome is circadian regulated i
 n different species. However\, we still lack a good understanding of the m
 echanisms involved in these genome-wide oscillations in gene expression. H
 ere we show that PSEUDO-RESPONSE REGULATOR 7 (PRR7)\, a central component 
 of the Arabidopsis clock\, is directly involved in the repression of maste
 r regulators of plant growth\, light signaling and stress responses. PRR7 
 forms a feedback loop with CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE EL
 ONGATED HYPOCOTYL (LHY) to regulate circadian rhythms. By combining chroma
 tin immunoprecipitation with next generation sequencing we could confirm t
 he direct regulation of CCA1 and LHY by PRR7 in addition to identifying th
 e genome-wide targets of PRR7. The expression levels of most PRR7 target g
 enes peak around dawn\, in an antiphasic manner to PRR7 protein levels and
  were repressed by PRR7. These findings indicate that PRR7 is important fo
 r cyclic gene expression by repressing the transcription of morning-expres
 sed genes. Furthermore\, using simple mathematical models we hypothesized 
 that the differences in the phase of expression of PRR7 target genes could
  be due to a combinatorial regulation with other cyclic factors. Using chr
 omatin immunoprecipitation and expression analysis we could show that inde
 ed several PRR7 targets are directly regulated by other clock components i
 n addition to PRR7.
LOCATION:Tom ap Rees\, Department of Plant Sciences
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