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SUMMARY:Sporduino: Parts for a Reliable and Field Ready Biosensing Platfor
 m - iGEM Team 2012
DTSTART:20130125T130000Z
DTEND:20130125T133000Z
UID:TALK42761@talks.cam.ac.uk
CONTACT:24938
DESCRIPTION:mplementation of biosensors in real world situations has been 
 made difficult by the unpredictable and non-quantified outputs of existing
  solutions\, as well as a lack of appropriate storage\, distribution and u
 tilization systems. This leaves a large gap between a simple\, functional 
 sensing mechanism and a fully realised product that can be used in the fie
 ld. We aim to bridge this gap at all points by developing a standardised r
 atiometric luciferase output in a Bacillus chassis. This output can be lin
 ked up with prototyped instrumentation and software for obtaining reliable
  quantified results. Additionally\, we have reduced the specialized requir
 ements for the storage and distribution of our bacteria by using Bacillus'
  sporulation system. To improve the performance of our biosensing platform
  we have genetically modified Bacillus’ germination speed. Lastly\, we d
 emonstrated the robustness of our system by testing it with a new fluoride
  riboswitch\, providing the opportunity to tackle real life problems.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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