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SUMMARY:Dynamics of single molecular motors labeled to quantum dots in a l
 iving cell - Sebastien Courty\, Laboratoire Kastler Brossel\, Dpartement d
 e Physique\, Ecole Normale Suprieure\, Paris
DTSTART:20051007T131500Z
DTEND:20051007T141500Z
UID:TALK4332@talks.cam.ac.uk
CONTACT:Duncan Simpson
DESCRIPTION:The study of molecular motors has greatly benefited\, at both 
 an \nexperimental and conceptual level\, from the development of \nsingle-
 molecule experiments. How the in vitro experiments relate to the \npropert
 ies and functional role of individual motors in living cells\, \nhowever\,
  remains unexplored\, largely due to a lack of experimental \nmethods to d
 etect individual cytoplasmic proteins in vivo. Here\, we \nmeasured the in
  vivo velocity and processivity distributions of \nindividual molecular mo
 tors (kinesin) labeled with quantum dots in the \ncytoplasm of living HeLa
  cells. By directly comparing the results with \nthose of in vitro motilit
 y assays\, we found that the kinesin motion was \nnot affected by the cond
 itions in the cytosol. Furthermore\, we observed \nthat an individual kine
 sin could travel over several microns through a \nsuccession of phases of 
 directed and diffusive motion. Our measurements \ndemonstrate the importan
 ce of single-molecule experiments in the \ninvestigation of intracellular 
 traffic and offer new prospects for the \nstudy of complex biological proc
 ess such as cell division.\n
LOCATION:Mott Seminar Room\, Cavendish Laboratory
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