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SUMMARY:Probing dynamic conformational changes in lipid signalling using h
 ydrogen deuterium exchangemass spectrometry  - Dr John Burke\, LMB\, Cambr
 idge U.K
DTSTART:20130327T101500Z
DTEND:20130327T111500Z
UID:TALK43844@talks.cam.ac.uk
CONTACT:23791
DESCRIPTION:Most cellular responses to extracellular stimuli have a common
  component of regulation arising from selective\nrecruitment of a network 
 of signalling complexes to membranes.  However\, studying these systems
  remains a daunting\ntask.  We have made unprecedented progress in unde
 rstanding these systems by applying a synthesis of hydrogen deuterium\nexc
 hange mass spectrometry (HDX-MS)\, X-ray crystallography and FRET spectros
 copy. Our application of this synthesis to\nphosphoinositide 3-kinase (PI3
 K) signalling represents one of the largest (> 200 kDa) complexes studied 
 using DXMS\, and\nelucidates novel aspects of PI3K regulation downstream o
 f GPCRs as well as a unifying mechanism of how diverse\noncogenic PI3K mut
 ants upregulate activity. 
LOCATION:Perham's seminar room
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