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SUMMARY:New tools for NGS libraries: Long-Span\, Mate-Pair Scaffolding and
  Methods for Faster Library Creation - David Mead\, Founder and Chief Exec
 utive Officer of Lucigen 
DTSTART:20130313T123000Z
DTEND:20130313T133000Z
UID:TALK43864@talks.cam.ac.uk
CONTACT:Laura Blackburn
DESCRIPTION:Large insert mate-pair reads have a major impact on the overal
 l success of de novo genome assembly and the discovery of inherited and ac
 quired structural variants. Molecular tools that bridge the gap between ma
 ssively parallel short read sequencing technologies (35-1\,500 bases) and 
 the need for large scaffolds (>40\,000 bases) to accurately assemble compl
 ex repeat-rich genomes are required. The NxSeq™ 40 kb Mate-Pair Cloning 
 Kit has been developed to facilitate genome assembly and gap closure. Resu
 lts show that approximately 40 kb paired-end sequences can be obtained by 
 either Illumina or 454 sequencing at an overall efficiency of 70%\, which 
 is significantly better than existing long-span\, mate-pair systems. This 
 presentation will describe the unique pNGS FOS vector\, which contains pri
 mer binding sites for next gen sequencing from Illumina or Roche 454 platf
 orms\, as well as the methodology with which to achieve long-span mate-pai
 r libraries for de novo genome assembly. In addition\, the NxSeq DNA Sampl
 e Prep Kits will be described\, which feature a combined end-repair and A-
 tailing master mix to enable significantly faster DNA library prep for NGS
  applications.\n\nDr. Mead will be joined by representatives of Cambridge 
 BioScience Limited\; Lara Spence\, Ella Popowicz and Mike Kerins.
LOCATION:CRUK Cambridge Institute Room 215
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