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SUMMARY:Characterization of function of Calmodulin-like (CML) 23 and CML24
  in the regulation of the Arabidopsis thaliana circadian clock - Nur Izzat
 i Mohd Noh
DTSTART:20130425T153000Z
DTEND:20130425T160000Z
UID:TALK44275@talks.cam.ac.uk
CONTACT:Megan Cooper
DESCRIPTION:CML23 and CML24 are potential Ca2+ sensors that are involved i
 n the regulation of the Arabidopsis thaliana circadian clock. These plant-
 specific family of Ca2+ sensor proteins may have overlapping roles in regu
 lating the Arabidopsis central circadian oscillator. A previous study prop
 osed that transcription of core oscillator genes are affected in the cml23
 -2cml24-4 double mutant. However\, how the circadian oscillator is regulat
 ed by CML23 and CML24 remains to be explained because CML23 and CML24 are 
 not thought to be transcription factors. It is likely that CML23 and CML24
  regulate the circadian oscillator through interacting intermediaries. Con
 formational changes undergone by CML23 and CML24\, as a consequence of Ca2
 + binding to the 4 EF hands\, may facilitate the recognition of target pro
 teins. In this study\, co-immunoprecipitation\, epistatic analysis and sub
 cellular localization are used to characterize the function of CML23 and C
 ML24 in the regulation of the Arabidopsis thaliana circadian clock. To ide
 ntify the putative interacting partners of CML23 and CML24\, Myc-tagged CM
 L23 and FLAG-tagged CML24 constructs were made and introduced into the Col
 -0 ecotype of Arabidopsis. In vivo co-imunoprecipitation of the transgenic
  lines will now be carried out. Database queries from www.ATTED-II have re
 sulted in identification of CML5 as a gene that is highly co-expressed wit
 h CML24. Circadian leaf movement rhythm of the cml5 mutant is being assess
 ed. In addition\, study of the epistatic relationship between core oscilla
 tor genes and cml23-2cml24-4 may indicate potential points of entry for CM
 L23 and CML24 into the circadian system. The known clock loss of function 
 mutants\, cca1-11\, lhy-21\, gi-11\, elf3-4\, elf4-1\, toc1-2\, ztl-3 and 
 lux-4 were crossed with cml23-2cml24-4 mutants. Circadian leaf movement rh
 ythm analysis will be used to screen for the phenotype of the triple mutan
 ts. We also hypothesize that CML23 and CML24 are cytosolic proteins\, whic
 h play a role in signal transduction from the cytosol into the nucleus. RF
 P-tagged CML23 and GFP-tagged CML24 transgenic plants that we have made wi
 ll be analysed to identify the subcellular localization of CML23 and CML24
 . 
LOCATION:Biffen Lecture Theater\, Department of Genetics
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