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SUMMARY:Controlling the distribution of signaling molecules in synthetic p
 atterning - Judith Savitskaya
DTSTART:20130628T110000Z
DTEND:20130628T112500Z
UID:TALK44310@talks.cam.ac.uk
CONTACT:Megan Cooper
DESCRIPTION:Natural biological processes like development and self-organiz
 ation of populations (ex: biofilm formation)\, require cells to respond to
  the distribution of a diffusing molecule. Synthetic biology has adapted a
 cyl homoserine lactone (AHL)-based quorum-sensing systems as a model for r
 ationally engineering intercellular communication\, chemotaxis\, and patte
 rn formation in bacterial populations. Many components of these synthetic 
 patterning systems have been characterized\, but little work has been done
  on characterizing and modifying the way that signaling molecules become d
 istributed in the field throughout space and time. We  used engineered C6-
 AHL receiver cells in order to ratiometrically characterize the rate of AH
 L diffusion in a bacterial lawn with different AHL sources. Using mathemat
 ical modeling based on experimental characterization of enzyme-catalyzed d
 egradation of C6-AHL by aiiA (an AHL lactonase)\, we show that aiiA can be
  used to control the shape and size of the AHL diffusion in a bacterial la
 wn.
LOCATION:Biffen Lecture Theater\, Department of Genetics
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