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SUMMARY:Phylogenetic shadowing guided by the cycling transcriptomes of two
  distantly related Brassicaeae species reveals the atlas of cis-regulatory
  elements of daytime-specific and daytime-unspecific pathways - Korbinian 
 Schneeberger (Max Planck Institute for Plant Breeding\, Cologne)
DTSTART:20131128T130000Z
DTEND:20131128T140000Z
UID:TALK47375@talks.cam.ac.uk
CONTACT:Ian Henderson
DESCRIPTION:Recent genome comparisons of a growing number of Brassicaceae 
 genome assemblies revealed the extent of conserved non-coding sequences (C
 NS). Despite these evolutionary constraints\, little is known about their 
 function. \nWe assembled the genome of Arabis alpina\, which diverged ~30 
 million years ago from A. thaliana and sequenced the transcriptomes of bot
 h species at 12 time points of two consecutive days. Over ~4\,000 ortholog
 ous genes showed daytime-dependent expression. \nComprehensively analyzing
  CNS for cis-regulatory elements revealed 50 putatively daytime specifying
  motifs\, including all those shown to be involved in diurnal expression s
 o far. Moreover\, absence or presence of many elements has significant imp
 act on the degree of conservation in gene expression\, evidencing their ro
 le in gene regulation.\nOn average diurnal genes are delayed by two hours 
 in A. alpina as compared to A. thaliana. The plant circadian clock can be 
 divided into three intervening loops\, but intriguingly this shift is only
  present in the morning and evening loops\, but not in the core loop. GO e
 nrichment and pathway analyses revealed striking differences between pathw
 ays conserved for their daytime and those being shifted. Conserved pathway
 s include photosynthesis and primary metabolite biosynthesis\, whereas shi
 fted pathways are enriched for secondary metabolites biosynthesis. 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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