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SUMMARY:Experimental Annotation of the Human Genome - Paul Bertone\, EMBL-
 European Bioinformatics Institute
DTSTART:20060215T150000Z
DTEND:20060215T160000Z
UID:TALK4773@talks.cam.ac.uk
CONTACT:Phil Cowans
DESCRIPTION:This talk will describe the development and application of gen
 omic DNA tiling arrays for the annotation of eukaryotic genomes\, with a p
 articular focus on homo sapiens. Tiling arrays are designed to represent a
 ll of the DNA comprising a chromosome or other genomic locus\, without pri
 or consultation of existing gene annotation. Because they are developed fo
 r the unbiased interrogation of genomic sequence\, tiling arrays enable th
 e discovery of novel transcripts and regulatory elements in eukaryotic gen
 omes. Two examples of microarray-based genome annotation are presented: la
 rge-scale mapping of transcribed sequences and identification of transcrip
 tion factor-binding sites. Chromosome- and genome-wide transcriptional act
 ivity is assessed by probing tiling arrays with normal complex tissue RNA\
 ; the locations of cis-regulatory elements are determined via array hybrid
 isation to chromatin-immunoprecipitated DNA\, originally bound in vivo by 
 a transcription factor of interest. These approaches are first demonstrate
 d with amplicon arrays representing all of the non-repetitive DNA of human
  chromosome 22\, then extended to the entire genome using maskless photoli
 thographic DNA synthesis technology. A large-scale tiling array survey rev
 ealed the presence of thousands of novel transcribed sequences in addition
  to known and predicted genes\, providing the first global transcription m
 ap of the human genome. 
LOCATION:Ryle Seminar Room\, Cavendish Laboratory
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