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SUMMARY:The substrate and function of mitochondrial ABC transporters invol
 ved in Fe-S metabolism - Dr Theresia Schaedler (Balk Group\, JIC)
DTSTART:20131129T130000Z
DTEND:20131129T132500Z
UID:TALK48383@talks.cam.ac.uk
CONTACT:Dr Yoan Coudert
DESCRIPTION:ABC transporters of the mitochondria (ATMs) are conserved in m
 any different species. In the model plant Arabidopsis thaliana\, ATM3 is r
 equired for cytosolic assembly of Fe-S clusters and is thought to provide 
 a so far unknown precursor for the cytosolic Fe-S cluster assembly pathway
 . Previous studies in Arabidopsis suggested that the transported compound 
 contains sulphur (but not iron) in an unknown chemical form (1). Additiona
 lly it was shown that glutathione (GSH) plays a role in the function of th
 e yeast orthologue Atm1 (2\, 3).\n\nThe aim of our study is to characteriz
 e the substrate specificity of plant AtATM3 and its functional orthologue 
 yeast Atm1. Using buthionine sulfoximine (BSO)\, an inhibitor of the first
  step of glutathione synthesis\, we assessed the effect of GSH depletion o
 n Arabidopsis atm3 mutants. We observed reduction of root growth in atm3 m
 utants in presence of low concentrations of BSO suggesting a role of GSH i
 n ATM function. To investigate the substrate specificity of ATMs further w
 e have established an expression system in the gram-positive bacterium Lac
 tococcus lactis\, and have purified ATMs in their functional form by affin
 ity purification. Using purified protein we screened for potential substra
 tes using ATP hydrolysis measurements and tested transport in inside-out v
 esicles. We also found a genetic link between ATM3 and the mitochondrial s
 ulphur dioxygenase ETHE1 suggesting the persulfide of glutathione is of ph
 ysiological relevance in the ATM transport reaction.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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